Hotspot

psbC-trnS-UGA

LSC · 11078-11237 · 160 bp

Back to Phalaris

Pi 0.0191

MarkerSeek score 65.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 3
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	346-349 bp	1.0	81.2
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.0	58.1	338-341 bp	1.0	81.0
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	339-342 bp	1.0	80.9
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	331-334 bp	1.0	80.9
psbC-trnS-UGA_p5	GCTGCAGCAGGTTTTGAAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.1	58.0	316-319 bp	1.0	80.8