Hotspot

ndhK

LSC · 50570-52080 · 1511 bp

Back to Phalaenopsis

Pi 0.0606

MarkerSeek score 68.80

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 1511
variable_sites: 204
pi: 0.0606
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CACACCGGGAGCGTAAATAA	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1232-1987 bp	0.857143	85.9
ndhK_p2	CACACCGGGAGCGTAAATAA	AAGGGTCTCCATCACTTCGA	50.0	50.0	58.0	58.1	1837-2592 bp	0.857143	85.7
ndhK_p3	TTGTACAAAGGAACCCTGCC	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1131-1771 bp	0.714286	80.2
ndhK_p4	TTGTACAAAGGAACCCTGCC	AAGGGTCTCCATCACTTCGA	50.0	50.0	58.0	58.1	1736-2376 bp	0.714286	80.0
ndhK_p5	TTGTACAAAGGAACCCTGCC	CACCACAAAAAGGGTCTCCA	50.0	50.0	58.0	57.9	1745-2385 bp	0.714286	80.0