Hotspot

psaC-ndhE

SSC · 115696-116418 · 723 bp

Back to Phalaenopsis

Pi 0.0498

MarkerSeek score 67.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 723
variable_sites: 88
pi: 0.0498
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTCCATCGGTTTCCAGTCTC	TTGTAGCTGCCACTCAGTTG	55.0	50.0	58.0	58.1	1642-1911 bp	0.357143	60.7
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	TTGTAGCTGCCACTCAGTTG	50.0	50.0	58.0	58.1	1177-1438 bp	0.357143	60.5
psaC-ndhE_p3	GTCCATCGGTTTCCAGTCTC	GTAGCTGCCACTCAGTTGTT	55.0	50.0	58.0	58.1	1640 bp	0.142857	44.7
psaC-ndhE_p4	GGACAAGCGGATTCACATCT	GTAGCTGCCACTCAGTTGTT	50.0	50.0	58.0	58.1	1175 bp	0.142857	44.5
psaC-ndhE_p5	TATACCAGAAAAGCCCGTGC	GTAGCTGCCACTCAGTTGTT	50.0	50.0	58.0	58.1	1358 bp	0.142857	44.5