Hotspot

ccsA-ndhD

SSC · 113886-114228 · 343 bp

Back to Phalaenopsis

Pi 0.0428

MarkerSeek score 70.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 343
variable_sites: 43
pi: 0.0428
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCAGCTCTTTTATGCGGAT	TTGCATTGCCAGGAATGAGT	45.0	45.0	58.0	58.1	969-1184 bp	0.785714	80.4
ccsA-ndhD_p2	ATGCAGCTCTTTTATGCGGA	TTGCATTGCCAGGAATGAGT	45.0	45.0	58.0	58.1	970-1185 bp	0.785714	80.3
ccsA-ndhD_p3	ATGCAGCTCTTTTATGCGGA	TCTGGACCACGAGAACTCTT	45.0	50.0	58.0	58.0	759-967 bp	0.642857	72.5
ccsA-ndhD_p4	TGCAGCTCTTTTATGCGGAT	TCTGGACCACGAGAACTCTT	45.0	50.0	58.0	58.0	758-966 bp	0.642857	72.5
ccsA-ndhD_p5	TTTTACCCAAGGTTTCGCCA	TCTGGACCACGAGAACTCTT	45.0	50.0	57.9	58.0	878-1086 bp	0.642857	72.0