Hotspot

rpoA-rps11

LSC · 80716-80798 · 83 bp

Back to Phalaenopsis

Pi 0.0386

MarkerSeek score 67.00

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 83
variable_sites: 9
pi: 0.0386
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	TTCAGATGTTGCACGTGTGA	GAACACCCTATGCTGCTCAA	45.0	50.0	58.1	57.9	478-506 bp	1.0	85.7
rpoA-rps11_p2	TCGCATTTCGAACAGGCATA	GAACACCCTATGCTGCTCAA	45.0	50.0	58.0	57.9	821-849 bp	1.0	84.3
rpoA-rps11_p3	TCGCATTTCGAACAGGCATA	AACACCCTATGCTGCTCAAG	45.0	50.0	58.0	57.9	820-848 bp	1.0	84.3
rpoA-rps11_p4	TCGCATTTCGAACAGGCATA	AAGAGATGCGGCATTACGAG	45.0	50.0	58.0	58.1	718-746 bp	1.0	84.3
rpoA-rps11_p5	TCGCATTTCGAACAGGCATA	GAAGAGATGCGGCATTACGA	45.0	50.0	58.0	58.1	719-747 bp	1.0	84.3