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PhapfoPt008-PhapfoPt009

LSC · 32550-33368 · 819 bp

Back to Phalaenopsis

Pi 0.0438
MarkerSeek score 78.90
Primer available yes
Label PhapfoPt008-PhapfoPt009

Feature Metadata

label_name
PhapfoPt008-PhapfoPt009
feature_type
igs
region
LSC
length_bp
819
variable_sites
97
pi
0.0438
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
PhapfoPt008-PhapfoPt009_p1 CGTTGCCTCCTTGAAAGAGA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.8 57.7 681-1068 bp 1.0 72.4
PhapfoPt008-PhapfoPt009_p2 TGTCCTGAACCACTAGACGA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.7 57.7 659-1046 bp 1.0 71.5
PhapfoPt008-PhapfoPt009_p3 CGTTGCCTCCTTGAAAGAGA CTACCACTGAGTTAAAAGGGC 50.0 47.6 57.8 56.3 680-1067 bp 1.0 68.7
PhapfoPt008-PhapfoPt009_p4 TGTCCTGAACCACTAGACGA CTACCACTGAGTTAAAAGGGC 50.0 47.6 57.7 56.3 658-1045 bp 1.0 67.8
PhapfoPt008-PhapfoPt009_p5 CCGTTGCCTCCTTGAAAGAG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 58.8 57.7 682-1069 bp 1.0 67.0