Hotspot

rpoB-PhapfoPt005

LSC · 27513-29045 · 1533 bp

Back to Phalaenopsis

Pi 0.0335

MarkerSeek score 71.30

Primer available yes

Label rpoB-PhapfoPt005

Feature Metadata

label_name: rpoB-PhapfoPt005
feature_type: igs
region: LSC
length_bp: 1533
variable_sites: 143
pi: 0.0335
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-PhapfoPt005_p1	CCAAATTAAGACCGCGGGTA	CCGGGGATAAAGGATTTGCA	50.0	50.0	58.0	57.9	1843-2029 bp	1.0	88.4
rpoB-PhapfoPt005_p2	CCAAATTAAGACCGCGGGTA	ACCGGGGATAAAGGATTTGC	50.0	50.0	58.0	57.9	1844-2030 bp	1.0	88.4
rpoB-PhapfoPt005_p3	CCAAATTAAGACCGCGGGTA	TCGAACCGGGGATAAAGGAT	50.0	50.0	58.0	58.2	1848-2034 bp	1.0	87.3
rpoB-PhapfoPt005_p4	CCCCTACACAGGCAAATTGT	CCGGGGATAAAGGATTTGCA	50.0	50.0	58.1	57.9	2282-2468 bp	1.0	86.6
rpoB-PhapfoPt005_p5	CCCCTACACAGGCAAATTGT	ACCGGGGATAAAGGATTTGC	50.0	50.0	58.1	57.9	2283-2469 bp	1.0	86.6