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PhapfoPt004-atpA

LSC · 10745-10915 · 171 bp

Back to Phalaenopsis

Pi 0.0553

MarkerSeek score 70.00

Primer available yes

Label PhapfoPt004-atpA

Feature Metadata

label_name: PhapfoPt004-atpA
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 26
pi: 0.0553
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
PhapfoPt004-atpA_p1	GGTTCAAATCCTATTGGACGC	CCGAAGAAGCTGAAACCCTT	47.6	50.0	57.3	58.1	255-326 bp	0.928571	73.8
PhapfoPt004-atpA_p2	GGTTCAAATCCTATTGGACGC	TCACCGAAGAAGCTGAAACC	47.6	50.0	57.3	58.1	258-329 bp	0.928571	73.6
PhapfoPt004-atpA_p3	TGGATAGGACAGAGGTCTTCT	CCGAAGAAGCTGAAACCCTT	47.6	50.0	56.9	58.1	290-361 bp	0.928571	70.1
PhapfoPt004-atpA_p4	TGGATAGGACAGAGGTCTTCT	TCACCGAAGAAGCTGAAACC	47.6	50.0	56.9	58.1	293-364 bp	0.928571	69.9
PhapfoPt004-atpA_p5	GTTCAAATCCTATTGGACGCA	CCGAAGAAGCTGAAACCCTT	42.9	50.0	56.6	58.1	254-325 bp	0.928571	68.2