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PhapfoPt003-PhapfoPt004

LSC · 10531-10672 · 142 bp

Back to Phalaenopsis

Pi 0.0625

MarkerSeek score 70.30

Primer available yes

Label PhapfoPt003-PhapfoPt004

Feature Metadata

label_name: PhapfoPt003-PhapfoPt004
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 25
pi: 0.0625
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
PhapfoPt003-PhapfoPt004_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	201-223 bp	1.0	75.3
PhapfoPt003-PhapfoPt004_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	204-226 bp	1.0	75.1
PhapfoPt003-PhapfoPt004_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	207-229 bp	1.0	74.7
PhapfoPt003-PhapfoPt004_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	210-232 bp	1.0	74.5
PhapfoPt003-PhapfoPt004_p5	CCAACGTCGACTATAACCCC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.8	56.9	222-244 bp	1.0	73.9