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PhapfoPt002-PhapfoPt003

LSC · 8443-9772 · 1330 bp

Back to Phalaenopsis

Pi 0.0833
MarkerSeek score 78.90
Primer available yes
Label PhapfoPt002-PhapfoPt003

Feature Metadata

label_name
PhapfoPt002-PhapfoPt003
feature_type
igs
region
LSC
length_bp
1330
variable_sites
364
pi
0.0833
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.88
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
PhapfoPt002-PhapfoPt003_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 871-1419 bp 1.0 63.7
PhapfoPt002-PhapfoPt003_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 870-1418 bp 1.0 63.7
PhapfoPt002-PhapfoPt003_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 874-1422 bp 1.0 62.6
PhapfoPt002-PhapfoPt003_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 873-1421 bp 1.0 62.6
PhapfoPt002-PhapfoPt003_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 872-1420 bp 1.0 62.6