Hotspot

psbI-PhapfoPt002

LSC · 8164-8354 · 191 bp

Back to Phalaenopsis

Pi 0.0503
MarkerSeek score 68.10
Primer available yes
Label psbI-PhapfoPt002

Feature Metadata

label_name
psbI-PhapfoPt002
feature_type
igs
region
LSC
length_bp
191
variable_sites
25
pi
0.0503
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.88
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-PhapfoPt002_p1 GTAATCCTGGGCGTGAAGAA GGGTTCGAATCCCTCTCTCT 50.0 55.0 57.6 58.2 194-237 bp 0.928571 84.2
psbI-PhapfoPt002_p2 GTAATCCTGGGCGTGAAGAA TTCGAATCCCTCTCTCTCCG 50.0 55.0 57.6 58.3 191-234 bp 0.928571 83.4
psbI-PhapfoPt002_p3 GTAATCCTGGGCGTGAAGAAT GGGTTCGAATCCCTCTCTCT 47.6 55.0 58.1 58.2 194-237 bp 0.928571 78.8
psbI-PhapfoPt002_p4 GTAATCCTGGGCGTGAAGAAT TTCGAATCCCTCTCTCTCCG 47.6 55.0 58.1 58.3 191-234 bp 0.928571 77.9
psbI-PhapfoPt002_p5 CGTAATCCTGGGCGTGAAG GGGTTCGAATCCCTCTCTCT 57.9 55.0 58.3 58.2 195-238 bp 0.928571 76.8