Hotspot

rps8

LSC · 82858-83253 · 396 bp

Pi 0.00415

MarkerSeek score 29.20

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 396
variable_sites: 4
pi: 0.00415
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	ATAAAACTGCGCCGGATTCT	AATCAAAGAAGCAGTGCCCA	45.0	45.0	58.0	58.0	1115-1124 bp	1.0	81.9
rps8_p2	ATAAAACTGCGCCGGATTCT	ATCAAAGAAGCAGTGCCCAA	45.0	45.0	58.0	58.0	1114-1123 bp	1.0	81.9
rps8_p3	ATAAAACTGCGCCGGATTCT	TGACAATGCAGCGGTTATCA	45.0	45.0	58.0	57.9	1001-1010 bp	1.0	81.2
rps8_p4	ATAAAACTGCGCCGGATTCT	TGATGACAATGCAGCGGTTA	45.0	45.0	58.0	57.9	1004-1013 bp	1.0	81.2
rps8_p5	ATAAAACTGCGCCGGATTCT	GTAGCGGATAACAGTGGAGC	45.0	55.0	58.0	58.1	1213-1222 bp	1.0	80.6