Hotspot

psaC-ndhE

SSC · 120617-121102 · 486 bp

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Pi 0.0319
MarkerSeek score 57.10
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
486
variable_sites
30
pi
0.0319
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 TGAAGAACCCGCGAATATGG ATCACAAGCCGAAGCATGAT 50.0 45.0 58.1 58.0 1172-1179 bp 0.375 55.0
psaC-ndhE_p2 AGCTCGAACACATTGGGTAC ATCACAAGCCGAAGCATGAT 50.0 45.0 57.9 58.0 789-796 bp 0.375 55.0
psaC-ndhE_p3 TTCTCGAGTACGGGCTTTTG ATCACAAGCCGAAGCATGAT 50.0 45.0 57.9 58.0 1042-1049 bp 0.375 54.9
psaC-ndhE_p4 TCGCGGATTTCGAGTAACTG ATCACAAGCCGAAGCATGAT 50.0 45.0 58.1 58.0 1448-1455 bp 0.375 54.5
psaC-ndhE_p5 TGGCCAAGCTGCTAAAGTAG ATCACAAGCCGAAGCATGAT 50.0 45.0 57.9 58.0 1430-1437 bp 0.375 54.4