Hotspot

ndhF-rpl32

SSC · 115425-116456 · 1032 bp

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Pi 0.0159
MarkerSeek score 53.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1032
variable_sites
33
pi
0.0159
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAGCGGAAAAAGGGGTATT TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.9 52.0 1116-1159 bp 1.0 42.9
ndhF-rpl32_p2 GGAGCGGAAAAAGGGGTATT TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.9 52.0 1117-1160 bp 1.0 42.9
ndhF-rpl32_p3 GGAGCGGAAAAAGGGGTATT TTTTTGACATAGAAGTACGTTT 50.0 27.3 57.9 52.0 1118-1161 bp 1.0 42.9
ndhF-rpl32_p4 ACATAGTAACTGGGAGCGGA TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.8 52.0 1128-1171 bp 1.0 42.9
ndhF-rpl32_p5 ACATAGTAACTGGGAGCGGA TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.8 52.0 1129-1172 bp 1.0 42.9