Hotspot

atpB-rbcL

LSC · 55139-56136 · 998 bp

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Pi 0.00726
MarkerSeek score 50.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
998
variable_sites
20
pi
0.00726
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 AGTAGTGGGATTGCTTCTCA GGTGACATAAGTCCCTCCCT 45.0 55.0 55.9 58.1 1039-1058 bp 1.0 66.0
atpB-rbcL_p2 TCAGAAGTAGTGGGATTGCT GGTGACATAAGTCCCTCCCT 45.0 55.0 55.9 58.1 1044-1063 bp 1.0 66.0
atpB-rbcL_p3 GAAGTAGTGGGATTGCTTCTCA GGTGACATAAGTCCCTCCCT 45.5 55.0 57.8 58.1 1041-1060 bp 1.0 65.6
atpB-rbcL_p4 TCAGAAGTAGTGGGATTGCTTC GGTGACATAAGTCCCTCCCT 45.5 55.0 57.8 58.1 1044-1063 bp 1.0 65.6
atpB-rbcL_p5 CAGAAGTAGTGGGATTGCTTCT GGTGACATAAGTCCCTCCCT 45.5 55.0 57.8 58.1 1043-1062 bp 1.0 65.5