Hotspot

ndhC-trnV-UAC

LSC · 50816-52153 · 1338 bp

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Pi 0.00964
MarkerSeek score 56.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1338
variable_sites
33
pi
0.00964
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.1 57.8 1394-1427 bp 1.0 49.9
ndhC-trnV-UAC_p2 AGGAATGCCCAGAAAATATCA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.9 57.8 1412-1445 bp 1.0 48.3
ndhC-trnV-UAC_p3 TCATATTCGTGAAGCAGAAACA AGCCCAATGCAACAAAATGC 36.4 45.0 56.1 58.5 1476-1509 bp 1.0 47.5
ndhC-trnV-UAC_p4 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.1 57.5 1393-1426 bp 1.0 47.1
ndhC-trnV-UAC_p5 AGGAATGCCCAGAAAATATCA AGCCCAATGCAACAAAATGC 38.1 45.0 54.9 58.5 1494-1527 bp 1.0 46.0