Hotspot

matK-trnK-UUU

LSC · 3265-4363 · 1099 bp

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Pi 0.0158
MarkerSeek score 62.80
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
1099
variable_sites
40
pi
0.0158
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCCTTGTAATTCTGCCATTT ATCCGGGTTGCTAACTCAAC 35.0 50.0 52.7 57.9 1116-1168 bp 1.0 46.8
matK-trnK-UUU_p2 TCCTTGTAATTCTGCCATTT AACGGTAGAGTACTCGGCTT 35.0 50.0 52.7 58.2 1099-1151 bp 1.0 46.8
matK-trnK-UUU_p3 TCCTTGTAATTCTGCCATTT ACGGTAGAGTACTCGGCTTT 35.0 50.0 52.7 58.2 1098-1150 bp 1.0 46.8
matK-trnK-UUU_p4 ATCCTTGTAATTCTGCCATT ATCCGGGTTGCTAACTCAAC 35.0 50.0 52.5 57.9 1117-1169 bp 1.0 46.8
matK-trnK-UUU_p5 TCCTTGTAATTCTGCCATTT GATCCGGGTTGCTAACTCAA 35.0 50.0 52.7 57.6 1117-1169 bp 1.0 46.8