Hotspot

ndhI

SSC · 116435-116935 · 501 bp

Back to Phaeostigma

Pi 0.501

MarkerSeek score 81.10

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 234
pi: 0.501
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TTTGTGACCTTTGAGGAGCC	AGACCACGGAAGTTACTCCA	50.0	50.0	58.0	58.0	1875 bp	0.5	59.8
ndhI_p2	GTACGGTTCTGCAATAGGGG	AGACCACGGAAGTTACTCCA	55.0	50.0	58.1	58.0	1835 bp	0.5	59.6
ndhI_p3	AATAGGGGTGGGAACAGTGA	AGACCACGGAAGTTACTCCA	50.0	50.0	57.9	58.0	1823 bp	0.5	59.5
ndhI_p4	GACCTTTGAGGAGCCGTATG	AGACCACGGAAGTTACTCCA	55.0	50.0	58.1	58.0	1870 bp	0.5	59.5
ndhI_p5	GATGGACTTTACCGAGGCTG	AGACCACGGAAGTTACTCCA	55.0	50.0	58.1	58.0	1364 bp	0.5	59.5