Hotspot

ndhH

SSC · 113015-114196 · 1182 bp

Back to Phaeostigma

Pi 0.466

MarkerSeek score 82.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 496
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TATGCAAACAACCCGATGCT	CCTGACATAAGAAGGCCGAC	45.0	55.0	58.2	58.1	2308 bp	0.5	58.2
ndhH_p2	TATGCAAACAACCCGATGCT	GACATAAGAAGGCCGACAGG	45.0	55.0	58.2	58.1	2305 bp	0.5	58.2
ndhH_p3	AGGTCTGCGGAAAATTCTGG	CCTGACATAAGAAGGCCGAC	50.0	55.0	58.2	58.1	1841 bp	0.5	58.1
ndhH_p4	AGGTCTGCGGAAAATTCTGG	GACATAAGAAGGCCGACAGG	50.0	55.0	58.2	58.1	1838 bp	0.5	58.1
ndhH_p5	GTCTGCGGAAAATTCTGGGA	GACATAAGAAGGCCGACAGG	50.0	55.0	58.2	58.1	1836 bp	0.5	58.1