Hotspot

ndhG

SSC · 117294-117824 · 531 bp

Back to Phaeostigma

Pi 0.486

MarkerSeek score 76.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 192
pi: 0.486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGTGAAGTATGTGTTCGCGT	TGGATTGACAGTGCCAAACT	45.0	45.0	57.9	57.6	1832 bp	0.5	55.9
ndhG_p2	TGTGAAGTATGTGTTCGCGT	GAGTCCAATAGCGGCTTCAG	45.0	55.0	57.9	58.4	1635 bp	0.5	55.5
ndhG_p3	TGTGAAGTATGTGTTCGCGT	AATAGCGAGTCCAATAGCGG	45.0	50.0	57.9	57.6	1641 bp	0.5	55.3
ndhG_p4	TCAAATTGCTTTGGGTCGGT	TGGATTGACAGTGCCAAACT	45.0	45.0	58.3	57.6	1617 bp	0.5	54.7
ndhG_p5	TCAAATTGCTTTGGGTCGGT	GAGTCCAATAGCGGCTTCAG	45.0	55.0	58.3	58.4	1420 bp	0.5	54.2