Hotspot

ycf1-rps15

SSC · 112245-112644 · 400 bp

Back to Phaeostigma

Pi 0.512

MarkerSeek score 76.50

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 400
variable_sites: 173
pi: 0.512
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATAATGGGAGCCGCTTGAG	CCGCAGACCTCTCTGAGATA	50.0	55.0	58.0	58.0	616 bp	0.5	59.6
ycf1-rps15_p2	AGAATCAGAATGGTGCGCAT	CCGCAGACCTCTCTGAGATA	45.0	55.0	58.0	58.0	807 bp	0.5	59.4
ycf1-rps15_p3	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	893 bp	0.5	59.3
ycf1-rps15_p4	AATAATGGGAGCCGCTTGAG	CCCTAATTGCTCTGGTGCAT	50.0	50.0	58.0	57.9	1088 bp	0.5	59.3
ycf1-rps15_p5	AGAATCAGAATGGTGCGCAT	AACACCATGCATTGATGGGT	45.0	45.0	58.0	58.1	1084 bp	0.5	59.1