Hotspot

EI075_pgp60

Genome · 738-1784 · 1047 bp

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Pi 0.452
MarkerSeek score 82.90
Primer available yes
Label EI075_pgp60

Feature Metadata

label_name
EI075_pgp60
feature_type
gene
region
Genome
length_bp
1047
variable_sites
437
pi
0.452
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp60_p1 TGGCCTAACAGAGCTTCTCT TGAGCTTCAGGTCCCCATAA 50.0 50.0 58.1 58.0 2002 bp 0.5 59.3
EI075_pgp60_p2 CGATCGGTCTCGTTTTAGCA TGAGCTTCAGGTCCCCATAA 50.0 50.0 58.1 58.0 2193 bp 0.5 59.0
EI075_pgp60_p3 TGGCCTAACAGAGCTTCTCT CTGACCAGCCTACGAAAACA 50.0 50.0 58.1 57.8 1467 bp 0.5 58.2
EI075_pgp60_p4 CGATCGGTCTCGTTTTAGCA CTGACCAGCCTACGAAAACA 50.0 50.0 58.1 57.8 1658 bp 0.5 57.9
EI075_pgp60_p5 GCTTTACCCTGGCCTAACAG TGAGCTTCAGGTCCCCATAA 55.0 50.0 58.2 58.0 2011 bp 0.5 57.7