Hotspot

EI075_pgp59

Genome · 1880-3283 · 1404 bp

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Pi 0.178
MarkerSeek score 82.10
Primer available yes
Label EI075_pgp59

Feature Metadata

label_name
EI075_pgp59
feature_type
gene
region
Genome
length_bp
1404
variable_sites
241
pi
0.178
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp59_p1 ATTCTGCTGCATCAGGATGG ATTCTTGATCACGACCACCG 50.0 50.0 58.0 58.0 2094 bp 0.5 59.8
EI075_pgp59_p2 CTGCTGCATCAGGATGGAAT ATTCTTGATCACGACCACCG 50.0 50.0 58.0 58.0 2091 bp 0.5 59.8
EI075_pgp59_p3 CTGGCTCAGGCAAATAGAGG ATTCTTGATCACGACCACCG 55.0 50.0 58.0 58.0 2173 bp 0.5 59.6
EI075_pgp59_p4 ATTCTGCTGCATCAGGATGG ATAGCTTCATGTGCTGGCTC 50.0 50.0 58.0 58.0 2712 bp 0.5 59.6
EI075_pgp59_p5 CTGCTGCATCAGGATGGAAT ATAGCTTCATGTGCTGGCTC 50.0 50.0 58.0 58.0 2709 bp 0.5 59.6