Hotspot

EI075_pgp42

Genome · 19190-21313 · 2124 bp

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Pi 0.247
MarkerSeek score 80.90
Primer available yes
Label EI075_pgp42

Feature Metadata

label_name
EI075_pgp42
feature_type
gene
region
Genome
length_bp
2124
variable_sites
507
pi
0.247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp42_p1 ATTTCCTCTTGCGCCTGAAA CCAAAACCACAAGTACCGGA 45.0 50.0 58.1 58.0 2793 bp 0.5 59.0
EI075_pgp42_p2 ATTTCCTCTTGCGCCTGAAA TGAAGCATTTGGATGTGCCT 45.0 45.0 58.1 58.1 2902 bp 0.5 58.7
EI075_pgp42_p3 TTCCTCTTGCGCCTGAAAAA CCAAAACCACAAGTACCGGA 45.0 50.0 58.3 58.0 2791 bp 0.5 57.3
EI075_pgp42_p4 TTTCCTCTTGCGCCTGAAAA CCAAAACCACAAGTACCGGA 45.0 50.0 58.3 58.0 2792 bp 0.5 57.3
EI075_pgp42_p5 TTCCTCTTGCGCCTGAAAAA TGAAGCATTTGGATGTGCCT 45.0 45.0 58.3 58.1 2900 bp 0.5 56.9