Hotspot

EI075_pgp24

Genome · 37181-38287 · 1107 bp

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Pi 0.214
MarkerSeek score 80.20
Primer available yes
Label EI075_pgp24

Feature Metadata

label_name
EI075_pgp24
feature_type
gene
region
Genome
length_bp
1107
variable_sites
234
pi
0.214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp24_p1 CCATCTGTCGCACTCATTGA CCAGGCGCTACTTTTCCATA 50.0 50.0 58.0 57.7 1889 bp 0.5 57.3
EI075_pgp24_p2 ACTTCACAAACCACTCGCAT CCAGGCGCTACTTTTCCATA 45.0 50.0 58.0 57.7 1832 bp 0.5 57.1
EI075_pgp24_p3 ACCGAACACAGAAACACCTC CCAGGCGCTACTTTTCCATA 50.0 50.0 58.1 57.7 2238 bp 0.5 57.0
EI075_pgp24_p4 CGAACACAGAAACACCTCCA CCAGGCGCTACTTTTCCATA 50.0 50.0 58.1 57.7 2236 bp 0.5 57.0
EI075_pgp24_p5 CACGTGTTCTTTCACCAACG CCAGGCGCTACTTTTCCATA 50.0 50.0 57.9 57.7 2260 bp 0.5 56.7