Hotspot

EI075_pgp21

Genome · 39107-40054 · 948 bp

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Pi 0.348
MarkerSeek score 78.20
Primer available yes
Label EI075_pgp21

Feature Metadata

label_name
EI075_pgp21
feature_type
gene
region
Genome
length_bp
948
variable_sites
248
pi
0.348
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp21_p1 TATGGAAAAGTAGCGCCTGG CACGTCGTGCTCAATTAGGA 50.0 50.0 57.7 58.0 1617 bp 0.5 57.3
EI075_pgp21_p2 TATGGAAAAGTAGCGCCTGG TATTCCATTCCGTCGTGCAG 50.0 50.0 57.7 58.1 1739 bp 0.5 56.9
EI075_pgp21_p3 TATGGAAAAGTAGCGCCTGG TTCCGTGATGTATTCGCTGG 50.0 50.0 57.7 58.1 1456 bp 0.5 56.9
EI075_pgp21_p4 TATGGAAAAGTAGCGCCTGG ATTCCGTCGTGCAGAATCAA 50.0 45.0 57.7 57.9 1733 bp 0.5 56.6
EI075_pgp21_p5 TATGGAAAAGTAGCGCCTGG ATGGTGTTGTTCGTGCTGAT 50.0 45.0 57.7 58.1 1758 bp 0.5 56.6