Hotspot

EI075_pgp02

Genome · 51586-52142 · 557 bp

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Pi 0.281
MarkerSeek score 80.20
Primer available yes
Label EI075_pgp02

Feature Metadata

label_name
EI075_pgp02
feature_type
gene
region
Genome
length_bp
557
variable_sites
153
pi
0.281
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp02_p1 GTGTTCCAGAAGTATACGGCT TGTCGGCAAAACAGCTAGTT 47.6 45.0 57.5 58.0 1539 bp 0.5 47.5
EI075_pgp02_p2 GTGTTCCAGAAGTATACGGCT TTGTCGGCAAAACAGCTAGT 47.6 45.0 57.5 58.0 1540 bp 0.5 47.5
EI075_pgp02_p3 GTGTTCCAGAAGTATACGGCT TTGCGTATTGTTCGTCGACT 47.6 45.0 57.5 57.9 1834 bp 0.5 47.4
EI075_pgp02_p4 GTGTTCCAGAAGTATACGGCT CCTGGTCAGCATGGTTCAAT 47.6 50.0 57.5 58.2 1765 bp 0.5 46.6
EI075_pgp02_p5 GTGTTCCAGAAGTATACGGCT TCTTGTCGGCAAAACAGCTA 47.6 45.0 57.5 57.8 1542 bp 0.5 45.8