Hotspot

EI075_pgp01

Genome · 52199-52810 · 612 bp

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Pi 0.276
MarkerSeek score 81.10
Primer available yes
Label EI075_pgp01

Feature Metadata

label_name
EI075_pgp01
feature_type
gene
region
Genome
length_bp
612
variable_sites
165
pi
0.276
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp01_p1 CCAGATCCTGTGCCACTAAC GGGACACCTGGTGGAAAATT 55.0 50.0 58.0 58.0 1226 bp 0.5 59.7
EI075_pgp01_p2 CTTCCTGCAGACGACCATAC GGGACACCTGGTGGAAAATT 55.0 50.0 58.1 58.0 1097 bp 0.5 59.3
EI075_pgp01_p3 CCAGATCCTGTGCCACTAAC GAGCGGTAGTACGTTCCAAG 55.0 55.0 58.0 58.1 1708 bp 0.5 59.0
EI075_pgp01_p4 CCAGATCCTGTGCCACTAAC ACTAGCGCCATGACAAAACA 55.0 45.0 58.0 58.1 1203 bp 0.5 58.9
EI075_pgp01_p5 CCAGATCCTGTGCCACTAAC ACCGAAAAATACACACCGCT 55.0 45.0 58.0 58.1 1567 bp 0.5 58.9