Hotspot

psbA

LSC · 296-1357 · 1062 bp

Back to Phacellaria

Pi 0.00314

MarkerSeek score 37.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTTTCAATAACTGGTAAACACT	ATAGAGACTGCGTGTGCTTG	30.4	50.0	54.1	58.0	1161 bp	1.0	40.3
psbA_p2	ACACTACTACTAAATAAGACGGA	ATAGAGACTGCGTGTGCTTG	34.8	50.0	54.1	58.0	1143 bp	1.0	40.3
psbA_p3	ACACTACTACTAAATAAGACGGAAG	ATAGAGACTGCGTGTGCTTG	36.0	50.0	55.9	58.0	1143 bp	1.0	40.3
psbA_p4	AACACTACTACTAAATAAGACGGA	ATAGAGACTGCGTGTGCTTG	33.3	50.0	54.8	58.0	1144 bp	1.0	40.3
psbA_p5	ACACTACTACTAAATAAGACGGAA	ATAGAGACTGCGTGTGCTTG	33.3	50.0	54.8	58.0	1143 bp	1.0	40.3