Hotspot

ccsA-psaC

SSC · 106182-107494 · 1313 bp

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Pi 0.0173

MarkerSeek score 64.50

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1313
variable_sites: 34
pi: 0.0173
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	CGGGCTGCATAGTTATGGTT	ACGAACTGAGGACTGTGTTG	50.0	50.0	58.0	57.8	1453-1459 bp	1.0	80.4
ccsA-psaC_p2	CGGGCTGCATAGTTATGGTT	CACGAACTGAGGACTGTGTT	50.0	50.0	58.0	57.8	1454-1460 bp	1.0	80.4
ccsA-psaC_p3	CGGGCTGCATAGTTATGGTT	AAGAGATGTGAATCCGCCTG	50.0	50.0	58.0	57.7	1428-1434 bp	1.0	79.7
ccsA-psaC_p4	TCTATTCGAAATCGGGCTGC	ACGAACTGAGGACTGTGTTG	50.0	50.0	58.1	57.8	1465-1471 bp	1.0	79.6
ccsA-psaC_p5	TCTATTCGAAATCGGGCTGC	CACGAACTGAGGACTGTGTT	50.0	50.0	58.1	57.8	1466-1472 bp	1.0	79.6