Hotspot

rrn4.5-rrn5

IRb · 102658-102865 · 208 bp

Back to Phacellaria

Pi 0.0128

MarkerSeek score 61.70

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 208
variable_sites: 3
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	GAGACGAGCCGTTTCTCATT	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	377-380 bp	1.0	81.0
rrn4.5-rrn5_p2	GAGACGAGCCGTTTCTCATT	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	375-378 bp	1.0	81.0
rrn4.5-rrn5_p3	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	322-325 bp	1.0	80.8
rrn4.5-rrn5_p4	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	320-323 bp	1.0	80.8
rrn4.5-rrn5_p5	GAGACGAGCCGTTTCTCATT	TCCCCTACAGTATCGTCACC	50.0	55.0	58.0	57.9	385-388 bp	1.0	80.7