Hotspot

psbE-petL

LSC · 60540-61802 · 1263 bp

Back to Phacellaria

Pi 0.0111

MarkerSeek score 64.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1263
variable_sites: 21
pi: 0.0111
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CCAACCTGCAATGAATAGGGA	AGTCGTATCTTGCTCATACCA	47.6	42.9	58.0	56.3	1456-1480 bp	1.0	51.2
psbE-petL_p2	AGCAAAAGAACGTTCTCCTGT	AGTCGTATCTTGCTCATACCA	42.9	42.9	58.1	56.3	1384-1408 bp	1.0	50.8
psbE-petL_p3	ACGTTCTCCTGTACTTCCAGA	AGTCGTATCTTGCTCATACCA	47.6	42.9	58.1	56.3	1375-1399 bp	1.0	50.4
psbE-petL_p4	TCAGCAAAAGAACGTTCTCCT	AGTCGTATCTTGCTCATACCA	42.9	42.9	57.8	56.3	1386-1410 bp	1.0	49.9
psbE-petL_p5	CGTTCTCCTGTACTTCCAGAC	AGTCGTATCTTGCTCATACCA	52.4	42.9	57.8	56.3	1374-1398 bp	1.0	49.7