Hotspot

trnT(GGU)-psbD

LSC · 31256-32430 · 1175 bp

Back to Phacellaria

Pi 0.00977

MarkerSeek score 66.40

Primer available yes

Label trnT(GGU)-psbD

Feature Metadata

label_name: trnT(GGU)-psbD
feature_type: igs
region: LSC
length_bp: 1175
variable_sites: 15
pi: 0.00977
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(GGU)-psbD_p1	GGCGTAAGTCATCGGTTCAA	GACCGGACCAACCTACAAAA	50.0	50.0	58.3	57.7	1256-1316 bp	1.0	80.6
trnT(GGU)-psbD_p2	GGCGTAAGTCATCGGTTCAA	GAAAGAGCAAGAGACCGGAC	50.0	55.0	58.3	58.3	1268-1328 bp	1.0	80.4
trnT(GGU)-psbD_p3	GGCGTAAGTCATCGGTTCAA	CTGCGTAACCAGTCATCCAT	50.0	50.0	58.3	57.7	1224-1284 bp	1.0	80.2
trnT(GGU)-psbD_p4	GGCGTAAGTCATCGGTTCAA	AGACCGGACCAACCTACAAA	50.0	50.0	58.3	58.6	1257-1317 bp	1.0	78.1
trnT(GGU)-psbD_p5	GCCATGGTAAGGCGTAAGTC	GACCGGACCAACCTACAAAA	55.0	50.0	58.7	57.7	1266-1326 bp	1.0	77.2