Hotspot

psbM-trnD(GUC)

LSC · 29039-29764 · 726 bp

Back to Phacellaria

Pi 0.0391

MarkerSeek score 78.70

Primer available yes

Label psbM-trnD(GUC)

Feature Metadata

label_name: psbM-trnD(GUC)
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 31
pi: 0.0391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD(GUC)_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	798-841 bp	1.0	65.4
psbM-trnD(GUC)_p2	TGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.6	58.5	805-848 bp	1.0	64.4
psbM-trnD(GUC)_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	799-842 bp	1.0	61.2
psbM-trnD(GUC)_p4	TGGAACTAGAATGAACAGTGCA	GTTCAATTGGTCAGAGCACC	40.9	50.0	57.6	57.0	806-849 bp	1.0	60.2
psbM-trnD(GUC)_p5	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.1	58.5	804-847 bp	1.0	59.7