Pi
0.0227
MarkerSeek score
68.60
Primer available
yes
Label
rps16-trnQ(UUG)
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps16-trnQ(UUG)_p1 | AAGTCGCACGTTGCTTTCTA | GAGGTTCGAATCCTTCCGTC | 45.0 | 55.0 | 58.1 | 58.1 | 1832-1867 bp | 1.0 | 84.3 |
| rps16-trnQ(UUG)_p2 | TGTCCTTCATTCAAGTCGCA | GAGGTTCGAATCCTTCCGTC | 45.0 | 55.0 | 57.5 | 58.1 | 1844-1879 bp | 1.0 | 81.0 |
| rps16-trnQ(UUG)_p3 | TCCTTCATTCAAGTCGCACG | GAGGTTCGAATCCTTCCGTC | 50.0 | 55.0 | 58.6 | 58.1 | 1842-1877 bp | 1.0 | 80.8 |
| rps16-trnQ(UUG)_p4 | TTCATTCAAGTCGCACGTTG | GAGGTTCGAATCCTTCCGTC | 45.0 | 55.0 | 57.3 | 58.1 | 1839-1874 bp | 1.0 | 80.1 |
| rps16-trnQ(UUG)_p5 | AAGTCGCACGTTGCTTTCTA | TTCGGAGGTTCGAATCCTTC | 45.0 | 50.0 | 58.1 | 57.3 | 1836-1871 bp | 1.0 | 79.5 |