Hotspot

matK-trnK(UUU)

LSC · 3470-4176 · 707 bp

Back to Phacellaria

Pi 0.0241
MarkerSeek score 65.90
Primer available yes
Label matK-trnK(UUU)

Feature Metadata

label_name
matK-trnK(UUU)
feature_type
igs
region
LSC
length_bp
707
variable_sites
18
pi
0.0241
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK(UUU)_p1 TGTTGCCGATATCTATCTCGT AACGGTAGAGTACTCGGCTT 42.9 50.0 56.4 58.2 775-778 bp 0.666667 46.7
matK-trnK(UUU)_p2 TGTTGCCGATATCTATCTCGT ACGGTAGAGTACTCGGCTTT 42.9 50.0 56.4 58.2 774-777 bp 0.666667 46.7
matK-trnK(UUU)_p3 TGTTGCCGATATCTATCTCGTT AACGGTAGAGTACTCGGCTT 40.9 50.0 57.2 58.2 775-778 bp 0.666667 44.4
matK-trnK(UUU)_p4 TGTTGCCGATATCTATCTCGTT ACGGTAGAGTACTCGGCTTT 40.9 50.0 57.2 58.2 774-777 bp 0.666667 44.4
matK-trnK(UUU)_p5 TGTTGCCGATATCTATCTCGT GGGTTGCTAACTCAACGGTA 42.9 50.0 56.4 57.5 788-791 bp 0.666667 44.3