Hotspot

ndhB

IRa · 143071-145282 · 2212 bp

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Pi 0.0312

MarkerSeek score 62.30

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRa
length_bp: 2212
variable_sites: 454
pi: 0.0312
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	TGGATGCAGTTACTAATTCACG	TTTGGGCGGAACAGATCTAC	40.9	50.0	56.5	57.6	2312 bp	0.083333	12.4
ndhB_p2	TGGATGCAGTTACTAATTCACG	TTGGGCGGAACAGATCTACT	40.9	50.0	56.5	58.4	2311 bp	0.083333	12.1
ndhB_p3	TGGATGCAGTTACTAATTCACG	TGGGCGGAACAGATCTACTA	40.9	50.0	56.5	57.6	2310 bp	0.083333	12.0
ndhB_p4	TTGGATGCAGTTACTAATTCACG	TTTGGGCGGAACAGATCTAC	39.1	50.0	57.2	57.6	2313 bp	0.083333	9.8
ndhB_p5	TTGGATGCAGTTACTAATTCACG	TTGGGCGGAACAGATCTACT	39.1	50.0	57.2	58.4	2312 bp	0.083333	9.5