Hotspot

ycf15-rps12

IRa · 140481-141452 · 972 bp

Back to Peucedanum

Pi 0.0183

MarkerSeek score 76.30

Primer available yes

Label ycf15-rps12

Feature Metadata

label_name: ycf15-rps12
feature_type: igs
region: IRa
length_bp: 972
variable_sites: 98
pi: 0.0183
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-rps12_p1	TCTCCTCGGTCCACAGATAC	GCAACTTTACGTAGGGCAGA	55.0	50.0	57.9	57.9	1089-1131 bp	0.916667	79.5
ycf15-rps12_p2	TTTCTCCTCGGTCCACAGAT	GCAACTTTACGTAGGGCAGA	50.0	50.0	58.1	57.9	1091-1133 bp	0.916667	79.4
ycf15-rps12_p3	TCTCCTCGGTCCACAGATAC	CGTAGGGCAGAGTTTGGTTT	55.0	50.0	57.9	58.1	1080-1122 bp	0.916667	79.4
ycf15-rps12_p4	TTTCTCCTCGGTCCACAGAT	CGTAGGGCAGAGTTTGGTTT	50.0	50.0	58.1	58.1	1082-1124 bp	0.916667	79.3
ycf15-rps12_p5	AAATGTAAGACTGGCGCCAA	GCAACTTTACGTAGGGCAGA	45.0	50.0	58.1	57.9	1068-1095 bp	0.916667	79.3