Hotspot

trnV-GAC-ycf15

IRa · 139608-140288 · 681 bp

Back to Peucedanum

Pi 0.0205

MarkerSeek score 61.60

Primer available yes

Label trnV-GAC-ycf15

Feature Metadata

label_name: trnV-GAC-ycf15
feature_type: igs
region: IRa
length_bp: 681
variable_sites: 88
pi: 0.0205
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-ycf15_p1	ACACTCTACCGCTGAGTTAT	GTACCGTGAGAGAAGCAAGG	45.0	55.0	55.7	58.0	789-807 bp	0.916667	59.6
trnV-GAC-ycf15_p2	ACACTCTACCGCTGAGTTAT	TGAGAGAAGCAAGGAGGTCA	45.0	50.0	55.7	58.0	783-801 bp	0.916667	59.5
trnV-GAC-ycf15_p3	TCTACCGCTGAGTTATATCCCT	GTACCGTGAGAGAAGCAAGG	45.5	55.0	57.6	58.0	785-803 bp	0.916667	59.0
trnV-GAC-ycf15_p4	ACACTCTACCGCTGAGTTAT	TACCGTGAGAGAAGCAAGGA	45.0	50.0	55.7	58.1	788-806 bp	0.916667	59.0
trnV-GAC-ycf15_p5	TCTACCGCTGAGTTATATCCCT	TGAGAGAAGCAAGGAGGTCA	45.5	50.0	57.6	58.0	779-797 bp	0.916667	58.9