Hotspot

ycf2-trnL-CAA

IRb · 92658-93993 · 1336 bp

Back to Peucedanum

Pi 0.085

MarkerSeek score 76.10

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1336
variable_sites: 321
pi: 0.085
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	CAGCCCTTTGTCTCTGTGTT	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	798-1967 bp	1.0	91.7
ycf2-trnL-CAA_p2	CAGCCCTTTGTCTCTGTGTT	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	800-1969 bp	1.0	91.7
ycf2-trnL-CAA_p3	GTTCTTGCAGAGTGGAACCA	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	922-2091 bp	1.0	91.7
ycf2-trnL-CAA_p4	GTTCTTGCAGAGTGGAACCA	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	924-2093 bp	1.0	91.7
ycf2-trnL-CAA_p5	GGCAAGCGGATCCTTTATGA	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	975-2144 bp	0.916667	88.3