Hotspot

petN-psbM

LSC · 28906-30060 · 1155 bp

Back to Peucedanum

Pi 0.0128

MarkerSeek score 71.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 90
pi: 0.0128
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	50.0	50.0	58.2	57.9	1303-1406 bp	1.0	86.7
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	50.0	50.0	57.6	57.9	1242-1345 bp	1.0	85.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	50.0	50.0	58.2	57.6	1302-1405 bp	1.0	84.3
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	50.0	50.0	57.6	57.6	1241-1344 bp	1.0	83.0
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	50.0	50.0	57.1	57.9	1304-1407 bp	1.0	80.7