Hotspot

rpoB-trnC-GCA

LSC · 26778-28006 · 1229 bp

Back to Peucedanum

Pi 0.0137

MarkerSeek score 68.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 71
pi: 0.0137
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1351-1374 bp	1.0	86.0
rpoB-trnC-GCA_p2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1352-1375 bp	1.0	86.0
rpoB-trnC-GCA_p3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1369-1392 bp	1.0	84.0
rpoB-trnC-GCA_p4	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1333-1356 bp	1.0	84.0
rpoB-trnC-GCA_p5	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1370-1393 bp	1.0	83.9