Hotspot

atpH-atpI

LSC · 13520-14485 · 966 bp

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Pi 0.0135

MarkerSeek score 70.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 75
pi: 0.0135
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	879-1280 bp	1.0	88.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	935-1336 bp	1.0	87.6
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	895-1296 bp	1.0	87.5
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	880-1281 bp	1.0	87.3
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.2	57.9	939-1340 bp	1.0	87.0