Hotspot

rpoA

LSC · 81114-82127 · 1014 bp

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Pi 0.00296

MarkerSeek score 51.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 3
pi: 0.00296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCTATTTTCACTAAGTAAGTCCA	AATGGCTGTAGACCTCCGAA	30.4	50.0	52.6	58.4	1144 bp	1.0	40.2
rpoA_p2	TCTATTTTCACTAAGTAAGTCCA	ATGGCTGTAGACCTCCGAAA	30.4	50.0	52.6	58.4	1143 bp	1.0	40.2
rpoA_p3	TTTCACTAAGTAAGTCCAATAGA	AATGGCTGTAGACCTCCGAA	30.4	50.0	52.6	58.4	1139 bp	1.0	40.2
rpoA_p4	TTTCACTAAGTAAGTCCAATAGA	ATGGCTGTAGACCTCCGAAA	30.4	50.0	52.6	58.4	1138 bp	1.0	40.2
rpoA_p5	TTTCACTAAGTAAGTCCAATAGA	TAATGGCTGTAGACCTCCGA	30.4	50.0	52.6	57.6	1140 bp	1.0	40.2