Hotspot

petD

LSC · 79696-80927 · 1232 bp

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Pi 0.00244

MarkerSeek score 52.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1232
variable_sites: 3
pi: 0.00244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCATGTCAACTAAACGGGGG	TCCCTAGATACACATGTCGT	50.0	45.0	57.5	55.1	1334-1336 bp	1.0	53.0
petD_p2	TCATGTCAACTAAACGGGGG	TTCCCTAGATACACATGTCGT	50.0	42.9	57.5	55.9	1335-1337 bp	1.0	51.6
petD_p3	TCATGTCAACTAAACGGGGG	GGGAGAATTCACTTTGAAGAACC	50.0	43.5	57.5	57.6	1360-1362 bp	1.0	49.4
petD_p4	TCATGTCAACTAAACGGGGG	ATTCCCTAGATACACATGTCGT	50.0	40.9	57.5	56.4	1336-1338 bp	1.0	47.9
petD_p5	TTCATGTCAACTAAACGGGGG	TCCCTAGATACACATGTCGT	47.6	45.0	58.2	55.1	1335-1337 bp	1.0	47.2