Hotspot

trnL-CAA-Ycf15

IRa · 146794-147141 · 348 bp

Back to Petunia

Pi 0.0115

MarkerSeek score 60.10

Primer available yes

Label trnL-CAA-Ycf15

Feature Metadata

label_name: trnL-CAA-Ycf15
feature_type: igs
region: IRa
length_bp: 348
variable_sites: 4
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-Ycf15_p1	AAATGGTAGACACGCGAGAC	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.0	58.0	474 bp	1.0	80.4
trnL-CAA-Ycf15_p2	TGAAATGGTAGACACGCGAG	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.0	58.0	476 bp	1.0	80.4
trnL-CAA-Ycf15_p3	AAATGGTAGACACGCGAGAC	GGAGAAGGAAGGCTGGAATG	50.0	55.0	58.0	58.0	473 bp	1.0	80.2
trnL-CAA-Ycf15_p4	TGAAATGGTAGACACGCGAG	GGAGAAGGAAGGCTGGAATG	50.0	55.0	58.0	58.0	475 bp	1.0	80.2
trnL-CAA-Ycf15_p5	GCCTTGGTGGTGAAATGGTA	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.1	58.0	486 bp	1.0	79.8