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petA-psbJ

LSC · 65913-67009 · 1097 bp

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Pi 0.00182

MarkerSeek score 59.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 2
pi: 0.00182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1156-1157 bp	1.0	75.8
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1158-1159 bp	1.0	75.8
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1155-1156 bp	1.0	74.9
petA-psbJ_p4	ACGTGTCCAAGGACTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1235-1236 bp	1.0	73.9
petA-psbJ_p5	TCCATTACGTGTCCAAGGAC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	1241-1242 bp	1.0	71.2