Hotspot

accD-psaI

LSC · 61314-62067 · 754 bp

Back to Petunia

Pi 0.00265

MarkerSeek score 60.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 2
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	949 bp	1.0	66.4
accD-psaI_p2	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.4	57.9	889 bp	1.0	66.3
accD-psaI_p3	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.4	57.9	948 bp	1.0	66.3
accD-psaI_p4	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.3	57.9	934 bp	1.0	65.8
accD-psaI_p5	TCCAGAAGGGTTTATTCGACC	TGCAATTGCCGGAAAGACTA	47.6	45.0	57.4	57.8	939 bp	1.0	65.7