Hotspot

trnT-UGU-trnL-UAA

LSC · 48527-49237 · 711 bp

Back to Petunia

Pi 0.00282

MarkerSeek score 60.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAAAAGCCAGCTATCGGAA	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	819-820 bp	1.0	78.0
trnT-UGU-trnL-UAA_p2	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	816-817 bp	1.0	77.3
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	771-772 bp	1.0	77.3
trnT-UGU-trnL-UAA_p4	GGAAAAGCCAGCTATCGGAA	CGTCTACCGATTTCGCCATA	50.0	50.0	58.0	57.6	813-814 bp	1.0	76.7
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	803-804 bp	1.0	76.4