Hotspot

ycf3-trnS-GGA

LSC · 46219-47058 · 840 bp

Back to Petunia

Pi 0.00238

MarkerSeek score 60.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 2
pi: 0.00238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	GCGAAGGTACGGAAAGAGAG	45.0	55.0	56.4	58.1	1032 bp	1.0	66.8
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	GCGAAGGTACGGAAAGAGAG	45.0	55.0	56.4	58.1	1033 bp	1.0	66.8
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1003 bp	1.0	66.7
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1004 bp	1.0	66.7
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	1024 bp	1.0	66.3